−254C>G SNP in the TRPC6 Gene Promoter Influences Its Expression via Interaction with the NF-κB Subunit RELA in Steroid-Resistant Nephrotic Syndrome Children

Figure 3

The TNF-α-mediated promoter activities are enhanced in podocytes and HEK 293T cells transfected with the reporter constructs containing the −254G allele. The TNF-α-mediated promoter activities are enhanced in podocytes and HEK 293T cells transfected with the reporter constructs containing the −254G allele. (a) In the TNF-α-mediated condition, the −254G allele (TNF-α-LUC1/2/3-M) significantly increased the luciferase activity of the TRPC6 gene in podocytes. (b) The results in HEK 293T cells were the same as that in (a). , , and ; luciferase activity of TNF-α-LUC1/2/3-M compared with that of LUC1/2/3-M.

(a)

(b)