−254C>G SNP in the TRPC6 Gene Promoter Influences Its Expression via Interaction with the NF-κB Subunit RELA in Steroid-Resistant Nephrotic Syndrome Children

Table 2

Primers designed for amplifying the promoter region of the TRPC6 gene used in the ChIP-PCR assay.

Primer	Length	Forward primer (5-3)	Reverse primer (5-3)
Primer 1	250 bp	CGAAAGCTCTATGAGGGCAGACA	CCACGTTTCCAAGCTGTTTTATT
Primer 2	252 bp	AACATCTGGCAGAGAAATCATTCTT	CCAGACTGCCTTCATTTTCTTTGGT
Primer 3	251 bp	AGGCTCGTGTCCCTTTTAAGGAACA	CAAGATCCCCACCTTTAAAGGGATC
Primer 4	250 bp	ACTAAATGAGCCAACTAAATAGTGG	GCGGAGAGCAAGGGAGACGGAGCTG
Primer 5	250 bp	TGAGGGCTGGAGAGTCTCTGTTGAC	AAGCAGGGGGTGCAGACGCCCGCCG