Genomic Analysis of a New Estrogen-Degrading Bacterial Strain, Acinetobacter sp. DSSKY-A-001

Table 2

PCR primer sequences.

Degradation enzyme	Primer sequence (5-3)	Annealing temperature (°C)	Length of the genes
Catechol 1,2-dioxygenase	F: ATGAACCGTCAACAAATTGATGC	55	907 bp
	R: GGCGTCGTTCAACTTCGCTAG
Dioxygenase	F: TGGTTCTCCCATGCTCGCTCT	55	700 bp
	R: ATTCCTAGAGCCCCCATAGAAAAAC
7α-Hydroxysteroid dehydrogenase	F: ATGTATCAGTCCCTATTAGATTTATCC	55	774 bp
	R: TTAATCAAGGGTTTGTACTCCG