Table 3: PCR mixture.

ComponentVolume

Template DNAμL
Primer F (10 μM)0.5 μL
Primer R (10 μM)0.5 μL
10x Easy Taq Buffer2.5 μL
2.5 mM dNTPsμL
DNA polymerase (Taq polymerase)0.5 μL
ddH2O18 μL
Total volume25 μL