Genotyping for Blast (Pyricularia oryzae) Resistance Genes in F₂ Population of Supa Aromatic Rice (Oryza sativa L.)

Table 3

Determination of the presence or absence of Pita and Pi9 genes for blast resistance and badh2 gene for aroma in F2 rice plants.

	Marker gene
	snpOS0006		snpOS0007b		snpOS0022
	Pita		Pi9		badh2
Plant	AL 1	AL 2	AL 1	AL 2	AL 1	AL 2
1A1	0	0	0	0	1	0
3B1	1	0	1	0	1	0
1C1	0	0	0	0	0	0
1D1	1	0	0	0	0	0
1 E1	1	0	0	0	0	0
3A2	0	0	0	0	1	0
1B2	1	1	0	0	1	0
1C2	1	1	0	0	1	0
3D2	1	0	0	0	1	1
3 E2	1	0	0	0	1	0
1F2	1	1	0	0	1	0
1G2	1	1	0	0	1	1
1H2	1	1	0	0	1	0
1A3	0	0	0	0	0	0
1B3	0	0	0	0	1	0
1C3	1	0	0	0	1	0
1D3	1	0	0	0	1	1
1 E3	1	0	0	0	1	1
1F3	0	0	0	0	1	0
1G3	1	0	0	0	1	0
1H3	0	0	0	0	0	0
1B4	1	0	0	0	0	0
1C4	0	0	0	0	0	0
1F4	0	0	0	0	1	0
1H4	0	0	0	0	1	0
1C5	1	1	0	0	1	1
1D5	1	1	0	0	1	1
3 E5	1	0	0	0	1	1
1H5	0	0	0	0	1	0
1A6	1	0	0	0	1	0
1B6	1	0	0	0	1	0
3C6	0	0	0	0	0	0
1 E6	0	0	0	0	0	0
1G6	1	0	0	0	1	0
1A7	1	0	0	0	1	1
1D7	1	1	0	0	1	0
1 E7	1	1	0	0	1	1
1H7	1	1	0	0	0	0
1A8	1	1	0	0	1	0
3 B8	0	0	0	0	1	0
1D8	1	0	0	0	0	0
3 E8	0	0	0	0	0	0
1F8	1	1	0	0	1	1
1H8	0	0	0	0	0	0
1C9	1	0	0	0	0	0
1G9	1	1	0	0	1	0
1H9	1	0	0	0	1	1
1A10	1	1	0	0	1	0
1B10	1	1	0	0	1	0
1C10	1	0	0	0	1	1
3D10	1	0	0	0	1	0
1 E10	1	0	0	0	0	0
1F10	1	1	0	0	1	1
3G10	1	1	0	0	1	0
1H10	1	1	0	0	1	0
1A11	1	0	0	0	1	1
1F11	1	1	0	0	1	0
1G11	0	0	0	0	1	1
1A12	1	0	0	0	1	0
1B12	1	1	0	0	1	1
1C12	1	1	0	0	1	1
1D12	1	0	0	0	1	0
1 E12	0	0	0	0	1	0
3G12	1	0	0	0	1	1
1B13	1	1	0	0	1	0
1C13	1	1	0	0	1	0
3D13	0	0	0	0	1	0
1 E13	1	1	0	0	1	1
1F13	1	1	0	0	1	0
1G13	1	1	0	0	1	0
1H13	0	0	0	0	1	0
2A1	0	0	0	0	1	0
2B1	1	1	0	0	1	0
2C1	0	0	0	0	1	1
2D1	1	0	0	0	0	0
4 E1	0	0	0	0	1	0
2H1	1	1	0	0	1	0
2A2	1	0	0	0	1	0
2B2	1	1	0	0	1	1
2C2	0	0	0	0	0	0
2D2	1	1	0	0	1	0
4 E2	1	0	0	0	0	0
2G2	1	1	0	0	1	1
2H2	1	1	0	0	1	1
2A3	0	0	0	0	1	0
4B3	1	1	0	0	1	0
2C3	1	0	0	0	1	1
2G3	1	1	0	0	1	0
2B4	1	1	0	0	1	0
4C4	0	0	0	0	1	0
2D4	0	0	0	0	1	1
2 E4	1	1	0	0	1	0
2G4	1	1	0	0	0	0
2H4	1	1	0	0	1	1
2A5	0	0	0	0	1	0
2F5	1	0	0	0	1	0
4G5	0	0	0	0	1	0
2H5	0	0	0	0	1	0
2A6	0	0	0	0	1	1
2B6	0	0	0	0	1	0
4 C6	0	0	0	0	1	0
2D6	0	0	0	0	1	0
2H6	1	1	0	0	1	0
Gigante	1	1	0	0	1	1
Vuninzara	1	1	0	0	1	0
Supa234	0	0	0	0	1	1
IRBL9-W	0	0	1	1	0	0
BC3	1	1	0	0	0	0
CO39	0	0	0	0	0	0

The plants with : 37 F₂ plants; resistant parent and control plants which were either resistant or highly resistant in both stages; others are moderately resistant and moderately susceptible. 1A1 to 2H6: F₂ plants; Gigante and Vuninzara: parent donor of Pita gene; Supa234: recipient parent with badh2 gene for aroma; IRBL9-W: Pi9 gene donor parent; BC3: positive control for Pita gene; CO39: negative control for all target genes (Pita, Pi9, and badh2) genes. AL 1: allele 1; AL 2: allele 2.