Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene

Figure 4

Epigenetic regulation of SOST in bone tissues. DNA methylation status of SOST promoter in three non-OPF and three OPF samples using sodium bisulfite sequencing. Each PCR product was subcloned and subjected to nucleotide sequencing analysis. (a) The percentage of methylated CpG sites in SOST promoter was calculated based on the BSP sequencing result. (b) BSP sequencing result of methylated CpG sites in each samples. Sequenced clones were depicted by filled (methylated) and open (unmethylated) circles for each CpG site.

(a)

(b)