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Target gene | Primer | Sequence (5-3)a | Restriction endonuclease | Vector | Annealing temperature (°C) | Amplicon size (bp) |
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floR | floR-F | GCTCTAGATTAGGGCGGCAGAGGGGGCTGGAAC | XbaI | pUCP24 | 64 | 1,477 |
floR-R | CCAAGCTTTTAGACGACTGGCGACTTCTCGGTG | HindIII |
catA2 | catA2-F | CGGAATTCAATAAAACCGGGCTTAATACAGATT | EcoRI | pUCP20 | 58 | 808 |
catA2-R | GCTCTAGATTATTTCAGTATGTTATCACACATC | XbaI |
blaCMY-97 | blaCMY-97-F | GCTCTAGATTATTATGGGTAGAAATATGCAAAT | XbaI | pUCP24 | 58 | 1,688 |
blaCMY-97-R | CCAAGCTTTTATTGCAGTTTTTCAAGAATGCGC | HindIII |
blaDHA-1 | blaDHA-1-F | GCTCTAGATAATCCACCTGTAAGTTTTTCTTTA | XbaI | pUCP24 | 58 | 1,267 |
blaDHA-1-R | CCAAGCTTTTATTCCAGTGCACTCAAAATAGCC | HindIII |
blaSHV-12 | blaSHV-12-F | GCTCTAGAATGGGTTCATGTGCAGCTCCATCAG | XbaI | pUCP24 | 58 | 1,023 |
blaSHV-12-R | CCAAGCTTTTAGCGTTGCCAGTGCTCGATCAGC | HindIII |
blaTEM-1b | blaTEM-1b-F | GCTCTAGAAGTATTGCCCGCTCCACGGTTTATA | XbaI | pUCP24 | 58 | 1,113 |
blaTEM-1b-R | CCAAGCTTTTACCAATGCTTAATCAGTGAGGCA | HindIII |
strA | strA-F | CGGAATTCCGGCCTGGTCCTTCAGCCACCATGC | EcoRI | pUCP20 | 58 | 1,590 |
strA-R | GCTCTAGATCAACCCCAAGTCAGAGGGTCCAAT | XbaI |
strB | strB-F | CGGAATTCGAACGAGAGCTACCGGTGCGGCTCG | EcoRI | pUCP20 | 58 | 1,167 |
strB-R | GCTCTAGACTAGTATGACGTCTGTCGCACCTGC | XbaI |
aac6 | aac6-F | CGGAATTCAGGTTGCCGGGTGACGCACACCGTG | EcoRI | pUCP20 | 58 | 913 |
aac6-R | GCTCTAGATTAGGCATCACTGCGTGTTCGCTCG | XbaI |
aac3 | aac3-F | CGGAATTCATCGCGATCCACGCTCAAACTGAAC | EcoRI | pUCP20 | 58 | 1,068 |
aac3-R | GCTCTAGATCAGGGCGAGCCAAAGTGCCGTTGA | XbaI |
aacc4cr | aacA4cr-F | CGGAATTCGGCGGTTTTCATGGCTTGTTATGAC | EcoRI | pUCP20 | 58 | 874 |
aacA4cr-R | GCTCTAGATTAGGCATCACTGCGTGTTCGCTCG | XbaI |
aac(6)-IIc | aac(6)-IIc-F | CGGAATTCACGCACACCGTGGAAACGGATGAAG | EcoRI | pUCP20 | 58 | 913 |
aac(6)-IIc-R | GCTCTAGATCATGACCACTTCCCCTTGATTTTG | XbaI |
qnrB4 | qnrB4-F | GGGGTACCCCCTACCGCTGGATCTGCGTGAATT | KpnI | pUCP24 | 62 | 901 |
qnrB4-R | CCAAGCTTTTAACCCATGACAGCGATACCAAGA | HindIII |
qnrB6 | qnrB6-F | CGGAATTCGCCAGCCTTTCATGATATATCTCCC | EcoRI | pUCP24 | 58 | 904 |
qnrB6-R | CGGGATCCCTAACCAATCACCGCGATGCCAAGC | BamHI |
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