Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles

Figure 4

Heat map representation of organ-specific CaASMT gene expression profiles in different tissues of pepper, including leaf (a), flower (b), pericarp (c), seed (d), and placenta (e). The data were normalized based on the mean expression value of each gene in all identified tissues. Then, the log2-transformed RPKM values were used to obtain heat maps using the software MeV 4.9.0. Red and green boxes indicate high and low expression levels of the CaASMT genes, respectively. Leaf samples were collected at 5, 10, 15, 20, 25, 30, 40, 50, and 60 days after emergence (DAE) and were named L1 to L9, respectively. Floral buds were collected at 0.25 cm, 0.35 cm, 0.5 cm, 0.7 cm, 0.8 cm, 1 cm, 1.2 cm, 1.45 cm, and 1.7 cm and were named F1 to F9, respectively. Placenta, seed, and pericarp grown from the tagged flowers were collected at 20, 25, 30, 35, 40, 45, 50, 55, and 60 days after flowering (DAF) and were named T1 to T9, S1 to S9, and G1 to G9, respectively.

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