Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop

Table 1

Raw data statistics and quality assessment.

Treatment/replicate	Total read bases (bp)	Total reads (pairs)	GC (%)	AT (%)	Q20 (%)	Q30 (%)
C1	3,682,737,548	18,231,374	44.77	55.23	97.08	95.08
C2	3,746,599,646	18,547,523	44.55	55.45	96.76	94.58
C3	3,881,717,446	19,216,423	44.49	55.51	97.0	94.98
D1	3,218,536,498	15933349	44.13	55.87	95.34	92.77
D2	3,711,544,566	18,373,983	44.8	55.2	96.97	94.93
D3	3,892,264,068	19,268,634	45.33	54.67	96.82	94.66
S1	3,775,903,180	18,692,590	44.58	55.42	97.06	95.04
S2	3,628,098,568	17,960,884	44.73	55.27	96.9	94.79
S3	3,481,866,526	17,236,963	44.32	55.68	96.9	94.82
H1	3,589,147,716	17,768,058	44.2	55.8	96.98	94.97
H2	3,541,905,774	17,534,187	44.11	55.89	96.92	94.93
H3	3,922,224,910	19,416,955	43.98	56.02	97.21	95.3
Total	44,072,546,446	218,180,923

Total read bases: total number of bases sequenced; total reads: total number of reads. In Illumina paired-end sequencing, read1 and read2 are added. GC (%): GC content; AT (%): AT content; Q20 (%): ratio of reads having a Phred quality score of over 20; Q30 (%): ratio of reads having a Phred quality score of over 30; C: control; D: drought stress; S: salinity stress; H: heat stress; numbers 1, 2, & 3 beside the treatment name: indicates biological replicate number.