Accumulation of Circulating Cell-Free CpG-Enriched Ribosomal DNA Fragments on the Background of High Endonuclease Activity of Blood Plasma in Schizophrenic Patients

Figure 2

Determination of rDNA CN in human cfDNA using NQH. (a) Scheme of the human ribosomal repeat. Segment of rDNA analyzed with NQH is shown. (b) (1) Photo of the membrane fragments with visualized rDNA. Three spots are applied for each DNA sample. The calibration DNA samples containing 640 (control 1) or 420 (control 2) copies of rDNA were put onto these spots of the filter. (2) The filter was scanned and the average integral intensity (±SD) of the spots was determined with software application “Imager 6.0.” (c) Dependence of on the total cfDNA concentration in the sample plotted for the standard samples (dashed lines) and for the tested DNA samples 1-9 (as an example). (2) The ratio was calculated. (d) Calibration dependence of rDNA CN on the ratio plotted for DNA calibration samples with known rDNA CN. (2) The rDNA CN in the cfDNA was calculated.

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