Diagnostic Value of miR-103 in Patients with Sepsis and Noninfectious SIRS and Its Regulatory Role in LPS-Induced Inflammatory Response by Targeting TLR4

Figure 3

miR-103 directly inhibited the expression of TLR4 in monocytes under LPS treatment. (a, b) Relative expression of TLR3 mRNA (a) and miR-103 (b) in monocytes treated with LPS (). (c) The predictive binding site of miR-103 on the 3-UTR of TLR4. (d) Luciferase activity results to confirm the interaction of miR-103 and TLR4 ( compared with the untransfected group). (e) Cell transfection efficiency examination results ( compared with the untransfected group). (f) Inhibiting effect of miR-103 on the mRNA expression of TLR4 in LPS-treated monocytes ( compared with the untransfected group). The data were collected from the experiments that were repeated at least three times.

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