circ_0002346 Suppresses Non-Small-Cell Lung Cancer Progression Depending on the Regulation of the miR-582-3p/STXBP6 Axis

Figure 1

circ_0002346 expression is downregulated in NSCLC tissues and cell lines. (a) The expression level of circ_0002346 was examined in 45 pairs of NSCLC tissues and paracancer tissues by RT-qPCR. (b) RT-qPCR was conducted to measure the level of circ_0002346 in the NSCLC cell lines (A549 and H1299) and the HBE cell line. (c) A schematic diagram of the genomic location and structural composition of circ_0002346 was shown. (d) The existence of circ_0002346 and CRIM1 from cDNA and gDNA was verified by PCR using the divergent and convergent primers, respectively. (e, f) The RNase R resistance of circ_0002346 and its host gene CRIM1 mRNA was analyzed by RT-qPCR. (g, h) The subcellular localization of circ_0002346 was analyzed, and 18S rRNA and U6 served as cytoplasmic and nuclear markers, respectively. .

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