circ_0002346 Suppresses Non-Small-Cell Lung Cancer Progression Depending on the Regulation of the miR-582-3p/STXBP6 Axis

Figure 2

Overexpression of circ_0002346 suppresses the malignant behaviors of NSCLC cells. (a–k) NSCLC cells were transfected with vector or circ_0002346 plasmid. (a) The expression of circ_0002346 was determined by RT-qPCR. (b) An EDU assay was performed to assess the rate of DNA synthesis by measuring the incorporation of EDU. (c) A colony formation assay was conducted to measure the colony number to analyze cell proliferation ability. (d, e) Transwell assays were conducted to measure the numbers of migrated and invaded cells to analyze cell migration and invasion abilities. (f) Flow cytometry was conducted to analyze the cell apoptosis rate (the percentage of NSCLC cells with FITC⁺ and PI^+/-). (g) The activity of caspase3 was analyzed using a caspase3 activity assay kit. (h) The expression of apoptosis-associated proteins (Bax and C-casp3) was measured in circ_0002346-overexpressed NSCLC cells by a Western blot assay. (i, j) The uptake of glucose and the production of lactate were determined using a fluorescence-based glucose/lactate assay kit. (k) A Western blot assay was conducted to measure the protein expression of HK2 and PKM2 in circ_0002346-overexpressed NSCLC cells. .

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