circ_0002346 Suppresses Non-Small-Cell Lung Cancer Progression Depending on the Regulation of the miR-582-3p/STXBP6 Axis

Figure 4

circ_0002346 overexpression-induced antitumor impacts are partly reversed by the addition of miR-582-3p mimics in NSCLC cells. (a) The transfection efficiency of miR-582-3p mimics in NSCLC cells was analyzed by RT-qPCR. (b–k) NSCLC cells were transfected with circ_0002346 alone or together with miR-582-3p mimics. (b, c) Cell proliferation ability was measured by an EDU assay and a colony formation assay. (d, e) Cell migration and invasion abilities were analyzed by transwell assays. (f) The apoptosis rate (FITC⁺ and PI^+/-) of NSCLC cells was assessed by flow cytometry. (g) Cell apoptosis was analyzed by measuring caspase3 activity using a caspase3 activity assay kit. (h) The protein expression of Bax and C-casp3 was detected by a Western blot assay. (i, j) The consumption of glucose and the level of lactate were analyzed using a fluorescence-based glucose/lactate assay kit. (k) The protein levels of HK2 and PKM2 were detected by a Western blot assay. ; .

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