circ_0002346 Suppresses Non-Small-Cell Lung Cancer Progression Depending on the Regulation of the miR-582-3p/STXBP6 Axis

Figure 6

STXBP6 silencing partly offsets circ_0002346 overexpression-induced effects in NSCLC cells. (a) A Western blot assay was conducted to measure the protein expression of STXBP6 in NSCLC cells transfected with si-NC or si-STXBP6. (b–k) A549 and H1299 cells were transfected with circ_0002346 alone or together with si-STXBP6. (b and c) An EDU assay and a colony formation assay were performed to measure cell proliferation ability. (d, e) Transwell assays were conducted to analyze cell migration and invasion abilities. (f) The apoptosis rate of NSCLC cells (FITC⁺ and PI^+/-) was analyzed by flow cytometry. (g) The activity of caspase3 was analyzed using a caspase3 activity assay kit. (h) The protein levels of Bax and C-casp3 were measured by a Western blot assay. (i, j) The uptake of glucose and the production of lactate were measured using a fluorescence-based glucose/lactate assay kit. (k) The protein expression of HK2 and PKM2 was examined in NSCLC cells by a Western blot assay. ; .

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