circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8

Figure 1

The expression level of circHUWE1 in NSCLC tissues and cells. (a) circHUWE1 was derived from the HUWE1 exon24. (b) PCR detected circHUWE1 and GAPDH using divergent primers and convergent primers in A549/DDP cells. (c) The relative expression of circHUWE1 was analyzed by RT-qPCR in adjacent non-tumorous tissues (), DDP-sensitive tissues (), and DDP-resistant tissues (). (d) RT-qPCR was used to assess circHUWE1 level in NSCLC cells and control BEAS-2B cells. (e–h) After treating with RNase R or actinomycin D, the relative RNA levels of circHUWE1 and HUWE1 mRNA were determined by RT-qPCR. (i, j) Cytoplasmic and nuclear fraction RNAs were isolated for measuring circHUWE1 level in cytoplasm and nuclei. .

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