circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8

Figure 2

Effects of circHUWE1 downregulation on DDP resistance, proliferation, apoptosis, cell cycle, migration, and invasion of NSCLC cells. (a–q) A549/DDP and H1299/DDP cells were transfected with sh-circHUWE1 or sh-NC. (a) The expression of circHUWE1 was assessed by RT-qPCR. (b–e) IC₅₀ of DDP in NSCLC cells was analyzed by the CCK-8 assay. (f) Colony forming assay was performed in A549/DDP and H1299/DDP cells. (g, h) The proliferation ability of A549/DDP and H1299/DDP cells was measured by the CCK-8 assay. (i, j) Cell apoptosis and cell cycle distribution were determined by flow cytometry. (k–n) Migrated and invaded cell number were analyzed by transwell assay. (o–q) The protein levels of PCNA, c-caspase 3, and MMP 13 were assessed by western blot assay. .

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