circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8

Figure 4

Knockdown of miR-34a-5p abolished circHUWE1-silencing-mediated effects on NSCLC cells. (a) RT-qPCR was employed to detect miR-34a-5p level in A549/DDP and H1299/DDP cells transfected with anti-miR-NC or anti-miR-34a-5p. (b–q) A549/DDP and H1299/DDP cells were transfected with sh-NC, sh-circHUWE1, sh-circHUWE1+anti-miR-NC, or sh-circHUWE1+anti-miR-34a-5p. (b) miR-34a-5p level was assessed by RT-qPCR. (c–f) CCK-8 assay was applied to test IC₅₀ of DDP in A549/DDP and H1299/DDP cells. (g–i) The proliferation ability of A549/DDP and H1299/DDP cells was examined by colony forming and CCK-8 assays. (j–l) Flow cytometry was implemented to analyze cell apoptosis and cell cycle distribution. (m, n) Transwell assay was conducted in A549/DDP and H1299/DDP cells. (o–q) Western blot assay was used to assess levels of PCNA, c-caspase 3, and MMP 13. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)

(q)