QTL Mapping of Traits Associated with Dual Resistance to the African Stem Borer (Busseola fusca) and Spotted Stem Borer (Chilo partellus) in Sorghum (Sorghum bicolor)

Table 12

QTL analyses at the three sites from the RIL sorghum population derived from .

Pest species	Site	Trait	QTL	Chr.	Position (cM)	Marker and loci interval	Supp interval	LOD		Additive effects	SE	Adj
B. fusca	Embu	DH	1	1	22	SB261_1 251-220	20-26	3.27	7.5	35.13	10.154
			2	3	24	DB103_3 1527-1528	22-26	4.11	9.6	-7.79	1.680
			3	5	10	AD323_5 2718-2834	8_12	3.97	11.2	8.86	1.731	28.3
		LD	1	2	4	CS115_2 936-927	2_6	3.11	9.1	-6.48	2.335
			2	2	84	CS190_2 1011-1172	82-86	3.55	8.1	8.13	1.821
			3	2	134	CS389_2 1210-1041	132-136	3.09	7.1	5.27	1.888
			4	2	148	CS414_2 1235-1083	146-150	3.67	8.7	-8.01	1.687
			5	10	30	CPS162_10 4621-4623	26-36	3.26	7.7	7.04	2.323	40.6
		EH	1	7	26	GH89_7 3380-3379	24-28	3.98	9	-1.42	0.337	9.0
		ST	1	7	22	GH87_7 3378-3376	20-24	3.51	8.2	-2.39	0.584
			2	9	72	BF123_9 4190-4193	70-74	3.48	8.1	-7.33	1.910	16.3
		WX	1	3	10	DB39_3 1463-1461	2-12	3.65	14.5	0.471	0.105
			2	3	56	DB155_3 1579-1590	54-58	3.17	7.3	0.578	0.112
			3	3	144	DB214_3 1638-1637	140-146	3.35	8.1	-2.180	0.469
			4	6	24	EF297_6 3158-3157	22-26	3.26	7.4	-3.888	1.085
			5	9	46	BF101_9 4168-4169	40-48	3.03	7.0	0.844	0.258	29.8
		VG	1	2	70	CS341_2 1162-1168	68-72	3.51	8.0	-0.394	0.101
			2	2	70	CS341_2 1162-1168	68-72	4.60	10.5	-0.423	0.092
			3	2	140	CS403_2 1224-1064	138-142	3.07	7.1	0.969	0.277
			4	5	10	AD323_5 2718-2834	8-12	3.04	8.7	-0.451	0.077
			5	6	60	EF325_6 3186-3073	58-62	3.06	7.0	-1.578	0.502
			6	7	56	GH99_7 3390-3391	52-60	5.53	12.4	-1.588	0.323
			7	7	58	GH100_7 3391-3394	52-60	5.18	11.6	-1.867	0.414
			8	7	58	GH100_7 3391-3394	52-60	5.00	11.3	-2.342	0.329	76.6
		TR	1	2	44	CS129_2 950-953	40-48	3.27	7.5	3.000	1.814
			2	2	64	CS139_2 960-962	62-66	3.21	7.5	-3.732	0.862
			3	6	52	EF320_6 3181-3068	50-54	3.83	8.8	-14.970	3.786	23.8
		GL	1	5	14	AD443_5 2838-2676	12-16	3.08	7.5	-0.696	0.225
			2	6	62	EF211_6 3072-3194	60-64	3.24	7.4	-0.841	0.221	14.9
B. fusca	Kabete	DH	1	1	28	SB691_1 681-208	26-30	3.53	8.1	12.95	3.602
			2	2	138	CS402_2 1223-1224	136-140	3.34	7.6	-7.2	2.177
			3	2	154	CS259_2 1080-1099	152-168	4.54	14.6	8.1	1.615
			4	2	82	CS350_2 1171-1011	80-84	3.19	7.3	-8.52	2.359
			5	3	70	DB172_3 1596-1603	68-72	8.16	17.5	-6.08	1.140
			6	3	74	DB169_3 1593-1592	72-78	3.91	9	-5.32	1.229
			7	4	58	BC149_4 2249-2250	52-60	3.29	7.7	5.77	1.590
			8	8	40	JK399_8 4047-3979	38-42	4.38	9.9	-20.68	5.332
			9	9	42	BF97_9 4164-4168	38-46	4.96	11.2	9.67	2.373
			10	9	56	BF106_9 4173-4179	52-58	5.19	11.7	-14.34	3.455	80.6
		LD	1	2	140	CS403_2 1224-1064	138-142	3.01	7	-9.54	2.926
			2	2	8	CS111_2 932-933	6_10	5.05	12.6	6.56	0.863
			3	3	132	CS397_2 1218-1219	130-134	5.41	12.1	4.15	0.902
			4	4	64	DB164_3 1588-1598	62-66	4.53	10.2	-3.37	0.834
			5	6	72	EF334_6 3195-3079	70-74	3.3	7.6	-14.42	4.207
			6	8	18	m08/014.9 3924-3929	14-22	3.17	7.7	-4.3	3.193
			7	10	12	CPS158_10 4617-4618	6_16	3.24	9.6	4.07	1.032	66.8
		EH	1	2	62	CS342_2 1163-964	60-64	4.26	9.6	1.17	0.266
			2	4	84	CS190_2 1011-1172	82-86	4.89	10.9	-1.32	0.284
			3	4	72	AD25_5 2420-2343	70-74	7.38	16.5	-2.29	0.434
			4	5	28	AD185_5 2580-2701	26-30	3.27	7.8	1.05	0.323
			5	6	4	EF255_6 3116-3143	2_6	3.67	8.4	-0.89	0.250
			6	9	88	BF138_9 4205-4203	86-90	3.25	7.5	-1.75	0.568
			7	8	44	JK208_8 3856-3959	42-46	3.7	8.4	-7.28	0.96
			8	9	86	BF137_9 4204-4205	84-88	7.13	15.6	-6.49	0.878	84.7
		ST	1	1	18	SB466_1 456-453	16-20	3.48	8.1	2.7	0.736
			2	2	52	CS132_2 953-954	48-58	3.27	7.5	-1.9	0.531
			3	2	54	CS133_2 954-955	48-58	3.2	7.4	-2.01	0.563
			4	2	90	CS150_2 971-1177	88-92	3.99	9.1	-2.3	0.597
			5	3	30	DB140_3 1564-1547	28-32	3.97	4.49	2.68	0.550
			6	7	22	GH87_7 3378-3376	20-24	3.04	7.1	-2.1	0.644
			7	3	32	DB143_3 1567-1568	30-34	6.17	13.9	3.98	0.515
			8	4	46	BC140_4 2240-2229	42-50	3.64	8.5	2.98	0.868
			9	7	74	GH108_7 3399-3420	72-76	3.48	8.2	-4.53	1.351	74.29
		WX	1	3	36	DB146_3 1570-1571	34-40	5.26	11.9	-0.797	0.069
			2	5	20	AD290_5 2685-2683	18-22	4.01	9.4	-0.337	0.078
			3	7	52	GH99_7 3390-3391	50-58	5.43	12.3	0.922	0.219	33.6
		VG	1	3	58	DB163_3 1587-1588	56-60	3.03	6.9	0.142	0.040	6.9
		TR	1	3	70	DB172_3 1596-1603	68-72	3.16	7.3	2.171	0.660
			2	6	16	EF184_6 3045-3161	12-18	3.06	7.1	-2.307	0.721
			3	9	72	BF123_9 4190-4193	70-74	7.22	16	-24.470	3.946	30.4
		GL	1	2	104	CS156_2 977-1188	102-106	3.51	8.0	-0.240	0.070
			2	3	4	DB40_3 1464-1463	2-6	4.17	15.9	0.139	0.029
			3	3	76	DB169_3 1593-1592	74-82	3.20	7.3	0.091	0.028
			4	6	60	EF325_6 3186-3073	58-62	5.62	12.5	0.874	0.196
			5	9	60	BF112_9 4179-4191	58-62	4.19	9.6	-0.193	0.052	53.3
C. partellus	Kiboko	DH	1	2	116	CS369_2 1190-1197	114-118	3.62	8.6	3.32	0.916
			2	2	134	CS389_2 1210-1041	132-136	4.99	11.7	-2.71	0.622
			3	6	56	EF322_6 3183-3184	54-58	3.49	8.3	-3.17	0.762
			4	9	106	BF152_9 4219-4220	104-108	3.13	12.6	-8.46	.815	41.2
		LD	1	2	56	CS133_2 954-955	52-58	3.84	8.7	-3.07	0.758
			2	2	132	CS397_2 1218-1219	130-134	3.78	8.6	-3.06	0.804
			3	6	16	EF184_6 3045-3161	14-18	3.14	7.2	2.76	0.801	24.5
		EH	1	3	50	DB153_3 1577-1580	42-52	3.99	9.1	0.59	0.153
			2	3	134	DB208_3 1632-1635	130-136	3.67	8.5	-1.01	0.287
			3	4	40	BC222_4 2322-2239	38-42	3.12	7.2	-0.89	0.255
			4	5	16	m05/015.6 2567-2709	14-18	3.6	8.4	-8.04	2.229
			5	2	148	CS414_2 1235-1083	146-150	3.04	7.4	0.61	0.183
			6	6	4	EF255_6 3116-3143	2_6	3.21	7.6	-0.55	0.172
			7	7	16	GH66_7 3357-3367	14-18	4.05	10.3	-0.51	0.148	58.5
		ST	1	3	42	DB152_3 1576-1573	40-46	5.08	11.4	2.64	0.391
			2	7	24	GH70_7 3361-3371	22-26	3.4	7.8	-1.83	0.471
			3	7	90	GH118_7 3409-3404	88-94	3.99	9.5	-3.56	0.837	28.7
		WX	1	2	154	CS259_2 1080-1099	152-168	3.88	13.5	-0.631	0.126	13.5
		VG	1	2	122	CS380_2 1201-1205	120-126	3.84	8.7	0.216	0.055
			2	6	22	EF303_6 3164-3158	20-24	3.04	7.1	1.546	0.490
			3	7	76	GH108_7 3399-3420	74-78	4.18	9.8	-1.036	0.264
			4	8	20	JK281_8 3929-3930	18-22	3.61	8.6	-0.259	0.068
			5	8	20	JK281_8 3929-3930	16-22	3.58	8.7	-0.279	0.073
			6	8	20	JK281_8 3929-3930	16-22	4.00	9.6	-0.266	0.073	52.5
		GL	1	2	104	CS156_2 977-1188	102-106	3.51	8.0	-0.240	0.070
			2	3	4	DB40_3 1464-1463	2-6	4.17	15.9	0.139	0.029
			3	3	76	DB169_3 1593-1592	74-82	3.20	7.3	0.091	0.028
			4	5	28	AD185_5 2580-2701	26-30	3.15	7.6	-0.168	0.046
			5	6	60	EF325_6 3186-3073	58-62	5.62	12.5	0.874	0.052
			6	6	60	EF325_6 3186-3073	58-62	5.12	11.6	0.867	0.230
			7	6	60	EF325_6 3186-3073	58-62	3.19	7.5	0.838	0.223
			8	7	22	GH87_7 3378-3376	18-24	3.55	8.3	0.159	0.038
			9	7	36	GH90_7 3381-3382	30-40	3.24	7.5	-0.328	0.110
			10	7	76	GH108_7 3399-3420	74-78	3.27	7.6	-0.541	0.147
			11	9	48	BF102_9 4169-4171	44-50	4.08	9.4	-0.287	0.083
			12	9	60	BF112_9 4179-4191	58-62	4.19	9.6	-0.193	0.052	112.8

QTL=quantitative trait loci; Chr=chromosome; cM=centimorgan; Position=maximum peak in cM, relative to the first locus on each chromosome; Supp interval=supportive interval; LOD=likelihood-ratio test statistic; Additive effect=regression coefficient of the QTL at the specific position from the multiple regression analysis. Positive additive effects indicate that the PB 15520-1 (resistant parent) allele increased the value of the trait; SE=standard error; =coefficient of determination between the respective QTL and the phenotypic observations from the whole data set; Adj =adjusted genetic variance. and indicate that data are significant at the ≤0.05 and ≤0.01 probability levels, respectively; DH=deadheart damage; LD=leaf feeding damage; EH=exit holes; ST=stem tunneling; WX=bloom waxiness; VG=seedling vigour; TR=trichome density; GL=leaf glossiness. Marker names in the table were coded for ease of analyses, and their full names are in the supplementary material (see).