Whole-Genome Sequencing Revealed a Late-Maturing Isogenic Rice Koshihikari Integrated with Hd16 Gene Derived from an Ise Shrine Mutant

Figure 2

Genetic procedure to identify a candidate SNP for late maturity derived from a cultivar Isehikari. (a) Genetic analyses for late maturity through F₂ and F₃ between Koshihikari×Isehikari and the backcross BC_1~3F₂ by using Koshihikari as a recurrent parent with the latest F₃ line in Koshihikari×Isehikari as a nonrecurrent parent. The late maturity derived from Isehikari was inherited as a single Mendelian factor. (b) Molecular linkage analysis by using late-maturing homozygous F₂ segregants of Koshihikari×Isehikari by 66 SSR markers distributed on 12 rice chromosomes. The late-maturing gene was linked to the SSR markers on the long arm of chromosome 3. (c) Development of late-maturity isogenic Koshihikari by continuous backcrossing for NGS analysis. (d) Whole-genome analysis of isogeneic lines (BC₄F₂ and BC₆F₂) integrated with the late-maturing gene in the Koshihikari genome.