Whole-Genome Sequencing Revealed a Late-Maturing Isogenic Rice Koshihikari Integrated with Hd16 Gene Derived from an Ise Shrine Mutant

Figure 6

Frequency distribution of SNPs per 0.1 Mb in the genome of late-maturing isogenic Koshihikari (BC₆F₂) based on whole-genome sequencing. There were less than 10 SNPs per 0.1 Mb over all genomes, except for a SNP cluster around the 33 Mb region of chromosome 3 (yellow bar graph), which contained over 15 SNPs per 0.1 Mb. This SNP-concentrated region derived from Isehikari was also detected by linkage analysis with SSR markers (Figure 1). A large portion of the 12 chromosomes were substituted with the genome sequence of Koshihikari after 6 times of continuous backcross targeting a late-maturing gene.