Molecular Characterization and Clinical Characteristics of m5C-Based RNA Methylation in Spinal Cord Injury: Validated by qPCR

Table 1

Messenger RNA-specific primers of hub genes.

Gene	Primer	Sequence (5—3)	Polymerase chain reaction products (bp)
Mx1	Forward	CTAAGCAGGAGACTGTGCCT	86
	Reverse	CAGGTGGATTCGGTTGGACA
Ifi35	Forward	CCAAAGTGGTTGAGCGGTTG	158
	Reverse	AAAGCCACTGACTAGCACCC
Col3a1	Forward	AGGGCAGGGAACAACTGATG	115
	Reverse	GGTCCCACATTGCACAAAGC
Irf7	Forward	GAAGTGAGGCCTGGGCTG	142
	Reverse	AAGATAAAGCGCCCTGTGCT
Usp18	Forward	TGAGAGGGTCCTTTCTGGCT	189
	Reverse	CTTCCTCCAGAGCTGAGTGC
Ifit2	Forward	GGCCATTGCAAACTACCGTC	93
	Reverse	GTGTTGTCAGGAGACAGGCT
Icam1	Forward	TTTCAGCTCCCATCCTGACC	144
	Reverse	GGGAAGTACCCTGTGAGGTG
Col1a1	Forward	GGAGAGAGCATGACCGATGG	184
	Reverse	GGGACTTCTTGAGGTTGCCA
Parp14	Forward	GTGCAGGCTGTGAAGAGAGT	141
	Reverse	GCCAAGGAAGGAGGCAATCT
Ddx60	Forward	TGACTCCGAAGCTTCCGCTA	188
	Reverse	CAGCATGCGCGTTAAGACAG
Oasl	Forward	TACTGCCAGACGCTCTTGTG	180
	Reverse	CAACCTGAAGCAGGGTCCAT
β-Tubulin	Forward	TAACGAGGCCCTCTACGACA	77
	Reverse	CGAGATGGTTCAGGTCTCCG