circPTN Promotes the Progression of Non-Small Cell Lung Cancer through Upregulation of E2F2 by Sponging miR-432-5p

Figure 2

Knockdown of circPTN inhibits the proliferation, migration, invasion, and tube formation ability of NSCLC cells. (a) Knockdown efficiency of sh-circTPN was detected by qRT-PCR. (b) CCK-8 proliferation assay in A549 and H1229 cells transfected with sh-NC or sh-circTPN (c) EdU incorporation assay in A549 and H1229 cells transfected with sh-NC or sh-circTPN. (d) Transwell-migration assay in A549 and H1229 cells transfected with sh-NC or sh-circTPN. (e) Transwell-invasion assay in A549 and H1229 cells transfected with sh-NC or sh-circTPN. (f) Tube formation assay in in A549 and H1229 cells transfected with sh-NC or sh-circTPN. (g) WB was performed to detect EMT-related proteins (E-cadherin, N-cadherin, and vimentin) in A549 and H1229 cells transfected with sh-NC or sh-circTPN. * ; **; and ***.

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