circPTN Promotes the Progression of Non-Small Cell Lung Cancer through Upregulation of E2F2 by Sponging miR-432-5p

Figure 3

circPTN targets miR-432-5p. (a) Three prediction software tools were used to predict the miRNA targets of circPTN. (b) RNA-pull down assay using biotin-labeled control (Bio-NC) or circTPN probe (Bio-circTPN) in A549 and H1229 cells. The relative enrichment of miR-432-5p and miR-326 was quantified by qRT-PCR. (c) The predicted binding sites between circPTN and miR-432-5p. (d) Dual-luciferase reporter assay in A549 and H1229 cells using WT reporter (with wildtype binding sites) or MUT reporter (with mutated binding sites) in the presence of miR-NC or miR-432-5p mimic. (e) RIP-qRT-PCR assay was performed in A549 and H1229 cells using anti-Ago2 or IgG. (f) qRT-PCR was used to detect the expression of miR-432-5p in 90 pairs of NSCLC tissues and adjacent normal tissues. (g) The correlation between circPTN and miR-432-5p expression levels in NSCLC tissues. *; **; and ***.

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