circPTN Promotes the Progression of Non-Small Cell Lung Cancer through Upregulation of E2F2 by Sponging miR-432-5p

Figure 4

circPTN regulates E2F2 protein expression through sponge miR-432-5p. (a) The binding sites between miR-432-5p and E2F2 mRNA were predicted by TargetScan. (b) Dual-luciferase reporter assay in A549 and H1229 cells using WT reporter (with wildtype binding sites) or MUT reporter (with mutated binding sites) in the presence of miR-NC or miR-432-5p mimic. (c) The protein level of E2F2 in A549 and H1229 cells upon miR-432-5p mimic transfection was detected by WB. (d) E2F2 protein levels in A549 and H1229 cells transfected with sh-NC, sh-circTPN, or sh-circTPN and miR-432-5p inhibitor were determined by WB. (e) qRT-PCR was used to detect the expression level of E2F2 in 90 pairs of NSCLC tissues and adjacent normal tissues. (f) The protein levels of E2F2 protein in three pairs of NSCLC tissues and corresponding adjacent tissues were determined by WB. (g) The correlation between the expression levels of E2F2 and miR-432-5p in NSCLC tissues. (h) The correlation between the expression levels of E2F2 and circPTN in NSCLC tissues. *; **; and ***.

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