ALDH1A3–Linc00284 Axis Mediates the Invasion of Colorectal Cancer by Targeting TGFβ Signaling via Sponging miR-361-5p

Figure 2

Knockdown of ALDH1A3 repressed CRC cell proliferation, migration, and invasion in vitro. (a) ALDH1A3 mRNA level in multiple CRC cell lines. (b) ALDH1A3 silencing efficiency in SW480 cells by shRNAs TRCN0000027144 (shorted to 44), TRCN0000027160 (shorted to 60), and TRCN0000027183 (shorted to 83) was verified by qPCR analysis. (c) The cell viability of ALDH1A3-silenced SW480 cells and the control cells was measured by CCK-8 assay. (d) Proliferation of SW480 cells was analyzed with Ki67 staining by flow cytometry. (e) The migration of ALDH1A3-silenced CRC cells was determined by wound healing assay. (f) The invasion of ALDH1A3-silenced CRC cells was analyzed by trans-well assay (3 wells per group).

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