LncRNA BANCR Promotes Endometrial Stromal Cell Proliferation and Invasion in Endometriosis via the miR-15a-5p/TRIM59 Axis

Figure 4

miR-15a-5p targets and inhibits TRIM59. (a) Downstream target genes of miR-15a-5p were predicted on the Starbase and miRWalk databases and intersections were identified; (b) Bindings sites of miR-15a-5p to TRIM59 were predicted on the Starbase database; (c) Binding relationship between miR-15a-5p and TRIM59 in Ect-ESCs was testified by the dual-luciferase assay; (d) Levels of TRIM59 in eutopic and ectopic endometrial tissues () were determined by RT-qPCR; (e)–(f) Expression levels of TRIM59 in Eut-ESCs and Ect-ESCs were determined by RT-qPCR (e) and Western blot assay (f); (g)–(h) Expression levels of TRIM59 in Ect-ESCs were determined by RT-qPCR (g) and Western blot assay (h); (i) Correlation between lncRNA BANCR and TRIM59 in ectopic endometrial tissues of EM patients () was analyzed by Pearson correlation analysis; (j) Correlation between miR-15a-5p and TRIM59 in ectopic endometrial tissues of EM patients () was analyzed by Pearson correlation analysis. Cell experiments were performed thrice independently. Data in figures (c), (e), and (h) were formalized as mean ± SD. Data in figure (c) were analyzed using two-way ANOVA, data in figures (d)–(f) were analyzed using the t test, and data in figures (g)–(h) were analyzed using one-way ANOVA, followed by Tukey’s multiple comparison test. ** .

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