Hepatocyte Lysosomal Membrane Stabilization by Olive Leaves against Chemically Induced Hepatocellular Neoplasia in Rats

Table 1

Proteolytic enzyme activities (μg-released tyrosine/mg substrate protein) and lysosomal integrity (total/free cathepsin-D activity) in liver tissue of rats treated by aqueous olive leaf extract (for one month) after trichloroacetic acid carcinogenic challenge (for 5 days). Values are expressed as mean ± S.E (𝑁=8).

	Group
Parameter	Control	TCA (500 mg/kg)	AOLE (500 mg/kg)	TCA + AOLE (500 mg/kg + 500 mg/kg)
Specific enzyme activity	24.2±0.55	24.9±0.32	24.4±0.39	24.7±0.3
Pepsin activity	15.5±0.13	15.8±0.13	15.3±0.13	12.3^∗∗±0.25
Trypsin activity	11.3±0.3	11.9±0.2	11.4±0.6	13.3^∗∗±0.2
Papain activity	18±1.1	22^∗±1.3	25^∗∗±0.9	24^∗∗±0.3
Free cathepsin-D activity	5.1±0.1	6.2^∗∗±0.2	5.5±0.2	4.5^∗±0.1
Total cathepsin-D activity	3.6±0.13	4±0.05	4±0.05	4.4^∗∗±0.04
Lysosomal integrity	0.72±0.03	0.65±0.02	0.74±0.03	0.99^∗∗±0.04

*Significantly different from control at ^∗∗𝑃<.05. Significantly different from control at 𝑃<.01.