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Figure 5: Alcohol and Hepcidin Expression. cDNA was synthesized from liver RNA of mice fed with regular (control) or ethanol-containing (alcohol) Lieber-De Carli liquid diets, as described in Materials and Methods. It was employed in real-time PCR to detect hepcidin expression. The mRNA expression in alcohol-fed mice was expressed as-fold of that in pair-fed control mice fed with regular diet (mean ± S.E.M.; , 4 mice per group). Asterisks indicate statistical significance ( ).