Combined Stimulation with the Tumor Necrosis Factor
and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices
Figure 3
Time course of caspase expression and activation. (a), Western blot analysis of caspase 8, 9 and 3 in normal liver (NL), core (C), after slicing (Sl), after pre-incubation (T0) and at the indicated times of culture. IETD- (b), LEHD- (c) and DEVD-AMC (d) caspase activities were measured in cell lysates from slices (open circles) and isolated hepatocytes (dark triangle) at different times of culture. Activities were expressed in arbitrary units (A.U.) of fluorescence. Caspase activities in isolated hepatocytes were measured in 6 independent experiments while activities in liver slices are the results of 3 independent experiments with 2 or 3 slices in each experiment. treatment versus control.
