Characterization of human umbilical cord-derived mesenchymal stem cells. (a) Immunofluorescent staining of HGF protein in the liver of HGF-UCMSC treated mice. No HGF was detected in livers from the control group. Cells were HGF-positive (red) two days after HGF adenovirus infection at MOI 50. (b) Western blot of HGF in the liver of treated mice. Ad-UCMSCs: UCMSCs transfected with adenovirus carrying the control vector. (c) Differentiation of human umbilical cord-derived mesenchymal stem cells. Osteogenic differentiation was detected by calcium deposits stained by Alizarin Red. Adipogenic differentiation was detected by oil red O staining (400×). Morphology of UCMSCs and HGF-UCMSCs at day 10 of culture (40x). (d-e) Mesenchymal stem cell marker profile of the parent UCMSCs and HGF-UCMSCs. The data are representative of three independent experiments.