A Combination of Leucine, Metformin, and Sildenafil Treats Nonalcoholic Fatty Liver Disease and Steatohepatitis in Mice
Met-Leu effects on hepatic fibrosis in mice. Mice were fed a low-fat (LF) diet or high-fat atherogenic (HC) diet for 6 weeks and then randomized to the indicated treatment groups for an additional 6 weeks. (a) Liver sections were stained with Picro Sirius Red for collagen at the end of the study (). Representative images for each group are shown. (b) Quantitation of Picro Sirius Red staining, calculated as % of the examined area. Data are expressed as mean ± SEM (). Sild: sildenafil, Met: metformin, and Leu: leucine. indicates groups which are not significantly different from LF.