A Combination of Leucine, Metformin, and Sildenafil Treats Nonalcoholic Fatty Liver Disease and Steatohepatitis in Mice
Sild-Met-Leu effects on fibrosis marker in mice. Mice were fed a low-fat (LF) diet or high-fat atherogenic (HC) diet for 6 weeks and then randomized to the indicated treatment groups for an additional 6 weeks. Gene expression of the collagens (a) Col1a1, (b) Col1a2, (c) Col4a1, and (d) transforming growth factor- (TGF-) beta was measured in liver extracts and expressed as mean ± SEM of fold change of control (). Sild: sildenafil, Met: metformin, Leu: leucine.