A Combination of Leucine, Metformin, and Sildenafil Treats Nonalcoholic Fatty Liver Disease and Steatohepatitis in Mice
Sild-Met-Leu treatment in hepatocytes decreases lipogenic gene expression. HepG2 cells were treated with sildenafil (Sild, 1 nM), metformin (Met, 0.1 mM), and leucine (Leu, 0.5 mM) as indicated for 24 hours after induction of lipid accumulation and compared to nontreated cells after lipid accumulation (high control) or without lipid accumulation (low control). (a) Fatty acid synthase (FAS), (b) stearoyl-coenzyme A desaturase 1 (SCD1), and (c) acetyl-coenzyme A carboxylase 1 alpha (ACC 1 alpha) gene expression were measured. Data are presented as mean ± SEM ( to 12). ((d) and (e)) Protein expression of FAS and SCD1: quantitative data, presented as mean ± SEM, and representative blots are shown ().