A Combination of Leucine, Metformin, and Sildenafil Treats Nonalcoholic Fatty Liver Disease and Steatohepatitis in Mice
Sild-Met-Leu treatment in hepatocytes increases PPAR alpha and delta and decreases SREBP1 and NF-κB. HepG2 cells were treated with sildenafil (Sild, 1 nM), metformin (Met, 0.1 mM), and leucine (Leu, 0.5 mM) as indicated for 24 hours after induction of lipid accumulation and compared to nontreated cells after lipid accumulation (high control) or without lipid accumulation (low control). Protein expression of (a) PPAR delta, (b) PPAR alpha, (c) sterol regulatory element-binding protein (SREBP) 1, and (d) ratio of phosphorylated to total NF-κB was measured. Quantitative data are presented as mean ± SEM () and representative blots are shown.