A Combination of Leucine, Metformin, and Sildenafil Treats Nonalcoholic Fatty Liver Disease and Steatohepatitis in Mice
Sild-Met-Leu effects on liver Kupffer cell activation in mice. Mice were fed a low-fat (LF) diet or high-fat atherogenic (HC) diet for 6 weeks and then randomized to the indicated treatment groups for an additional 6 weeks. (a) Liver sections were stained with anti-CD68 antibody at the end of the study (). Representative images for each group are shown. (b) Quantitation of CD68 staining, calculated as % of the examined area. Data are expressed as mean ± SEM (). Sild: sildenafil, Met: metformin, Leu: leucine.