Isoniazid and Rifampicin Produce Hepatic Fibrosis through an Oxidative Stress-Dependent Mechanism

Table 1

PCR primers.

Primer		Primer sequence
NOX1	Forward	5-CTGACAAGTACTATTACACGAGA-3
NOX1	Reverse	5-CATATATGCCACCAGATTAGGGA-3
NOX2	Forward	5-CTTTCTCAGGGGTTCCAGTG-3
NOX2	Reverse	5-TCTTCCAAACTCTCCGCAGT-3
NOX4	Forward	5-TGAGGAGTCACTGAACTA-3
NOX4	Reverse	5-TGACTGAGGTACAGCTGGA-3
p22^phox	Forward	5-TGGACGTTTCACACAGTGGT-3
p22^phox	Reverse	5-TAGGCTCAATGGGAGTCCAC-3
p47^phox	Forward	5-CACCGAGATCTACGAGTTCCA-3
p47^phox	Reverse	5-TGTCAAGGGGCTCCAGATAG-3
p67^phox	Forward	5-GCCACAGTCATGTTCAATGG-3
p67^phox	Reverse	5-ACAAAAGCCTTCGGGAAAAT-3
PDGF-Rβ	Forward	5-AGCACCTACCTGCCTCTGAA-3
PDGF-Rβ	Reverse	5-GCACGGCAGTGTAGAGAACA-3
α-SMA	Forward	5-ACTACTGCCGAGCGTGAGAT-3
α-SMA	Reverse	5-AAGGTAGACAGCGAAGCCAA-3
TGF-β	Forward	5-GTCAGACATTCGGGAAGCAG-3
TGF-β	Reverse	5-GCGTATCAGTGGGGGTCA-3
COL1A1	Forward	5-GAAACCCGAGGTATGCTTGA-3
COL1A1	Reverse	5-GACCAGGAGGACCAGGAAGT-3
TIMP-1	Forward	5-ATCAATGCCTGCAGCTTCTT-3
TIMP-1	Reverse	5-ATCTCCAAGTGCACAAGCCT-3
MMP2	Forward	5-GAGAAGGATGGCAAGTATGGC-3
MMP2	Reverse	5-GCGGTCTCGGGACAGAATCC-3
MMP9	Forward	5-GAGTGGACGCGACCGTAGTTGG-3
MMP9	Reverse	5-GTACATGAGCGCTTCCGGCACGC-3
β-Actin	Forward	5-TGGAATCCTGTGGCATCCATGAAAC-3
β-Actin	Reverse	5-TAAAACGCAGCTCAGTAACAGTCCG-3

NOX = NADPH oxidase; PDGF-Rβ = platelet-derived growth factor receptor β; α-SMA = α-smooth muscle actin; TGF-β = transforming growth factor β; COL1A1 = collagen 1A1; TIMP = tissue inhibitor of matrix metalloproteinase; MMP = matrix metalloproteinase.