Role of the Rostral Ventrolateral Medulla in the Arterial Hypertension in Chronic Renal Failure

Table 1

PCR primer sequences for Real Time quantitative PCR.

Gene	Primer	Sequence (5′ to 3′)	Product size (bp)	Annealing Temperature
AT1	5′	ATG-CCA-GTG-TGT-TTC-TGC-TC	244	58,0°C
		CCA-ATG-GGG-AGT-GTT-GAG-TT
β-actin	5′	CCT-CTA-TGC-CAA-CAC-AGT-GC	191	56,9°C
		ACA-TCT-GCT-GGA-AGG-TGG-AC
gp91^phox	5′	AGT-GGT-TCG-CAG-ACC-TGC-TG	239	57°C
		TCT-GGT-GTT-GGG-GTG-TTG-AC
p47^phox	5′	GTC-TGA-GGG-TGA-AGC-CAT-CG	211	59°C
		CCG-AGA-ACG-CTG-GTG-GAT-GC

Angiotensin II type 1 (AT₁), β-actin (housekeeping), NAD(P)H subunits (p47^phox and gp91^phox).