Cardiovascular Disease, Single Nucleotide Polymorphisms; and the Renin Angiotensin System: Is There a MicroRNA Connection?

Figure 3

The human AT₁R +1166 A/C SNP occurs in the miR-155-binding site. (a) Complementarity between miR-155 and the hAT₁R 3^′-UTR site targeted (70–90 bp downstream from the human AT₁R stop codon). The +1166 A/C SNP corresponds to the nucleotide 86 bp downstream from the human AT₁R stop codon (shown in red print). The binding of miR-155 to the hAT₁R 3^′-UTR target site fulfills the requirement of a 7-bp seed sequence of complementarity at the miRNA 5^′ end when the +1166 A-allele is expressed. (b) Complementarity between miR-155 and the human AT₁R 3^′-UTR harboring the +1166 C-allele. If the +1166 C-allele is expressed, the seed sequence requirement would not be met and, as a consequence, it would be expected that human AT₁R expression would be elevated [79].

(a)

(b)