Methods for Imaging Renin-Synthesizing, -Storing, and -Secreting Cells
Figure 2
Portions of the renal preglomerular vasculature comprised of an interlobular artery (ILA) and afferent arterioles (AA) were isolated after HCl hydrolysis in rats chronically treated with captopril (see legend to Figure 1). Renin cells are localized to the AA tip (single arrows); captopril treatment also recruits renin cells upstream (dashed arrows). (a) Single labeling against renin (see Figure 1 legend) with ABC peroxidase kit (brown label, Vector Laboratories, Burlingame, CA). Recruited renin cells are spindle-shaped as smooth muscle cells, whereas renin cells at the tip of AA adopt a more globular shape. (b) Double labeling against renin (green label, fluorescein isothiocyanate-labeled secondary anti body) and against -SM actin (red label, tetramethylrhodamine-labeled secondary antibody); for primary antibodies see legend to Figure 1. The image was generated as previously detailed [86] using a confocal microscope (Biorad MRC1024, Bio-Rad Life Science Research, Hercules, CA) by projecting 52 serial optical sections. Bars: 50 m.
