Polymorphisms in α- and β-Adrenergic Receptor Genes, Hypertension, and Obstructive Sleep Apnea: The Skaraborg Sleep Study

Table 1

PCR and digestion conditions for genotyping of the the 𝛼_2𝐵-, 𝛽₁-, and the 𝛽₂-adrenergic receptor gene polymorphisms.

		PCR conditions					RFLP		Gel^‡‡
Gene/polymorphism	Forward (sense) primer sequence
N successfully genotyped	Reverse (antisense) primer sequence	Size (bp)	Annealing T (^∘C)	Extension time (s)	Mg^2+ mmol/L	Cycles	Enzyme	Time (h)	%
338 A2BAR
I/D 301–303	5^′-AGGGTGTTTGTGGGGCATCT	112/103	72	30	1.5	33	—		3
	5^′-CAAGCTGAGGCCGGAGACACT
337* B1AR
Ser49Gly	5^′-CCGGGCTTCTGGGGTGTTCC	562	64	30	1.5	35	EcoO109I	3	2
	5^′-GGCGAGGTGATGGCGAGGTAGC
Arg389Gly	5^′-CGCTCTGCTGGCTGCCCTTCTTCC	530	64	30	1.5	33	BcgI	3	2
	5^′-TGGGCTTCGAGTTCACCTGCTATC
338 B2AR
Arg16Gly	5^′-CGCCTTCTTGCTGGCACGCAAT**	203	60	30	1.5	30	BsrDl	2	4.5
	5^′-CCAGTGAAGTGATGAAGTAGTT
Gln27Glu	5^′-CCGGACCACGACGTCACCCAG***	169	60	30	1.5	30	BstNl	2	4.5
	5^′-CCAGTGAAGTGATGAAGTAGTT

A2BAR: 𝛼_2B-adrenergic receptor gene; B1AR: 𝛽₁-adrenergic receptor; B2AR: 𝛽₂-adrenergic receptor; Ag: agarose; bp: base pair.
*The genotyping failed in one individual.
**The underlined nucleotide is a mismatch to create a BsrDl recognition site in case of the Gly16-allele.
***The underlined nucleotide is a mismatch to create a BstNl recognition site in case of the Glu27-allele.
^‡‡The PCR products were separated on a multipurpose agarose gel (Ag) with ethidium bromide and visualised with UV-light.