Recombinant Expression and Characterization of Human and Murine ACE2: Species-Specific Activation of the Alternative Renin-Angiotensin-System
Figure 1
Batch quality of recombinant human and murine ACE2. (a) Equal amounts (5 μg) rmACE2 and rhACE2 were analyzed by size-exclusion chromatography. The elution was continuously monitored by in-line measurement of the absorbance at 280 nm. The eluted peaks for purified rmACE2 (blue) and rhACE2 (red) are shown in the chromatogram and given in absorption units (mAU). (b) Equal amounts (7 μg) of rmACE2 (m) and rhACE2 (h) were subjected to SDS-PAGE analysis followed by in-gel protein staining with Coomassie Brilliant Blue. Selected marker bands are depicted in the graph to allow the estimation of molecular weights. (c) Equal amounts (2 μg) of rmACE2 (m) and rhACE2 (h) were analyzed in native PAGE followed by in-gel protein staining with Coomassie Brillant Blue as described in Section 2.
