Heme Oxygenase-1 Attenuates Hypoxia-Induced sFlt-1 and Oxidative Stress in Placental Villi through Its Metabolic Products CO and Bilirubin
Figure 2
HO-1 and bilirubin suppress hypoxia-induced increases in superoxide production. In response to culture in hypoxic conditions, there is a significant ~60% increase in the level of superoxide generated by the placental explants as assayed by dihydroxyethidium (DHE) fluorescence. Administration of CoPP had no significant effect on normoxic explants but completely attenuated the hypoxia-induced increase seen in the control explants. Exogenous application of bilirubin (Bili) had a significant attenuation of superoxide in both the normoxic and hypoxic samples when compared to their respective controls. Statistical significance at the level is indicated by “*” versus normoxic controls and “#” versus hypoxic controls.
