581780.fig.001a
(a)
581780.fig.001b
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581780.fig.001c
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Figure 1: Chromatography of human urine from normal and hypertensive subjects on DEAE-cellulose. (a1) Normotensive subjects with two peaks with ACE activity corresponding to 190 and 65 kDa ACE; (b1) Hypertensive subjects with two peaks with ACE activity corresponding to 90 and 65 kDa ACE; (c1) Normotensive subjects with three peaks with ACE activity corresponding to 190, 90, and 65 kDa ACE. The dialyzed human urine (100 mL) was applied to a DEAE-cellulose column (  cm). The column was washed with 20 mM Tris/HCl buffer, pH 7.0, and then eluted (fractions of 4.5 mL) with a linear gradient of 20 mM to 500 mM Tris/HCl buffer, pH 7.0, at a flow rate of 55 mL/h. (∘) Absorbance at 280 nm. (•) ACE activity with HHL as substrate. (□) Conductivity. Werstern blotting analysis of urinary ACEs. (a2) Normotensive subjects with 190 and 65 kDa ACE; (b2) Hypertensive subjects with 90 and 65 kDa ACE; (c2) Normotensive subjects with 190, 90, and 65 kDa ACE (as described in Section 2).