From Rat to Human: Regulation of Renin-Angiotensin System Genes by Sry

Figure 2

Variation at amino acid 76 of rat Sry proteins effects on rat RAS gene promoters. Amino acid variation between Sry1 and Sry3 results in differential activity on promoters of the RAS with Sry1 P76T (a) and Sry3 T76P (b). (a) Sry1 P76T significantly increased activity on Ren, Ace, and Ace2. (b) Sry3 T76P significantly decreased activity on Agt, Ace, and Ace2. Asterisks indicate significance from respective nonmutant Sry. ^∗𝑃≤0.05, ^∗∗𝑃≤0.01, ^∗∗∗𝑃≤0.001. Blue bars are the control pEF-1 vector and black bars represent proteins with a proline at amino acid 76.

(a)

(b)