From Rat to Human: Regulation of Renin-Angiotensin System Genes by Sry

Table 2

Cloning of the human renin-angiotensin system gene promoters. The start and stop are the base pairs from the transcriptional start site of each gene.

Gene	Restriction enzymes	Right primer	Left primer	Size (bp)	Stop	Start
AGT	NcoI/SacI	ATACCATGGGGCCACTTCTGACCCTGCTG	GCCGAGCTCTAGAAGATCCCCCAGCTGATAG	1610	34	−1576
REN	NcoI/MluI	GTTCCATGGGAGGTTCTGTGGCTCCCTTAG	CGCACGCGTCTTCTTATGGGAAGCCCATTTA	1452	8	−1444
ACE	BglII/KpnI	CAGAGATCTGTGCTCGGCTCTGCCCCTTCTC	CGAGGTACCCCAAGCTGTTAGGACCCCTGAG	1700	21	−1691
ACE 2	HindIII/NheI	CCGAAGCTTTCCTGATCCTCTGTAGCCATGGGA	CGAGCTAGCAGGGCAGGCAGCATCTGACT	1932	34	−1899
AT2r	NcoI/MluI	CCGCCATGGGTCCACTGGGAGCCTTCAACCT	CGAACGCGTGGTGGAGGTGAGGCGGCAAA	2906	3	−2903
Mas	HindIII/NheI	CCGAAGCTTCCATGAGGAGGCCTCAGGTTGGA	CGAGCTAGCGCCCGTTTGGACCTGGTCGC	2204	18	−2186