Reciprocal Effects of Oxidative Stress on Heme Oxygenase Expression and Activity Contributes to Reno-Vascular Abnormalities in EC-SOD Knockout Mice

Figure 4

(a) EETs levels in WT and EC-SOD^(−/−) mice and mice treated with CoPP. EETs levels were determined as described in Methods. Results are mean ± SE, 𝑛=4; *𝑃<0.05 versus WT mice, ^‡𝑃<0.05 versus EC-SOD^(−/−) and ⁺𝑃<0.05 versus WT + CoPP mice. (b) Adiponectin levels in WT and EC-SOD^(−/−) mice and mice treated with CoPP. Adiponectin levels were determined as described in methods. Results are mean ± SE, 𝑛=4; *𝑃<0.05 versus WT mice, ^‡𝑃<0.05 versus EC-SOD^(−/−) mice, and ⁺𝑃<0.05 versus WT + CoPP mice. (c) AKT and pAKT proteins in arteries of WT and EC-SOD^(−/−) mice. Mice were injected with CoPP once a week as described in Methods. Blots are representative of four separate experiments. Results are expressed as mean ± SE, *𝑃<0.05 versus WT, ^‡𝑃<0.01 versus EC-SOD^(−/−), ⁺𝑃<0.05 versus WT + CoPP mice.

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