Deficiency of ACE2 in Bone-Marrow-Derived Cells Increases Expression of TNF-α in Adipose Stromal Cells and Augments Glucose Intolerance in Obese C57BL/6 Mice

Table 2

Primers used in this study.

Gene	Primers
F4/80	Forward 5′-CTTTGGCTATGGGCTTCCAGTC-3′; Reverse 5′-GCAAGGAGGACAGAGTTTATCGTG-3′
TNF-α	Forward 5′-CCCACTCTGACCCCTTTACTC-3′; Reverse 5′-TCACTGTCCCAGCATCTTGT-3′
TNF-α receptor	Forward 5′-CAGTCTGCAGGGAGTGTGAA-3′; Reverse 5′-CACGCACTGGAAGTGTGTCT-3′
CCR2	Forward 5′-AGAGAGCTGCAGCAAAAAGG-3′; Reverse 5′-GGAAAGAGGCAGTTGCAAAG-3′
CCL2	Forward 5′-CCTGCTGCTACTCATTCACC-3′; Reverse 5′-TGTCTGGACCCATTCCTTCT-3′
IL-1beta	Forward 5′-ATCTGGGATCCTCTCCAGCCAAGC-3′; Reverse 5′-AAAGGTTTGGAAGCAGCCCTTCAT-3′
PAI-1	Forward 5′-ACTGCAAAAGGTCAGGATCG-3′; Reverse 5′-ACAAAGGCTGTGGAGGAAGA-3′
Mgl-1	Forward 5′-ATGATGTCTGCCAGAGAACC-3′; Reverse 5′-ATCACAGATTTCAGCAACCTTA-3′
IL-4	Forward 5′-AGAAGGGACGCCATGCACGG-3′; Reverse 5′-ATGCGAAGCACCTTGGAAGCCC-3′
YM-1	Forward 5′-GACCTGCCCCGTTCAGTGCC-3′; Reverse 5′-TGCCAGTCCAGGTTGAGGCCA-3′
Mrc-1	Forward 5′-AGGGCAAGCTGCAAGCAGCA-3′; Reverse 5′-CCCACTGCCAACCACTGCGT-3′