Heme Oxygenase-1 Induction and Organic Nitrate Therapy: Beneficial Effects on Endothelial Dysfunction, Nitrate Tolerance, and Vascular Oxidative Stress
Figure 5
Effects of organic nitrates on the human HO-1 promoter activity. DLD1-HO-11kb-Prom cells were treated for 8 h with isosorbide dinitrate ISDN, PETN, isosorbide-5-mononitrate ISMN, or GTN at a concentration of 50 μM or the respective solvent (DMSO, H2O, EtOH). Extracts were prepared, and luciferase activity and protein content were measured, and luciferase activity was normalized to the protein content. Shown (mean ± SEM; = 8–10) are the normalized luciferase activity values. The normalized luciferase activity of the cell treated with solvent was set to 100%. (***= , ns: not significant different from solvent-treated cells.) These data were partly published in [34].
