Oxidative Stress and Heme Oxygenase-1 Regulated Human Mesenchymal Stem Cells Differentiation
Evaluation of lipid content measured as percentage of Oil Red O staining. (a) Representative photographs demonstrating an increase in lipid droplets in the bone marrow of obese mice compared to lean mice and obese mice treated with CoPP. (b) Quantitative analysis of bone marrow lipid content showing a significant increase in Oil Red O staining in the obese vehicle. Please note bars on the graphs represent the mean ± SEM of three independent experiments; * versus vehicle-treated lean mice; # versus vehicle-treated ob mice. (c) HO activity of bone marrow cells showing an increase in HO activity in both lean mice and obese mice treated with CoPP when compared to obese mice not receiving treatment. Please note bars on the graphs represent the mean ± SEM of four independent experiments; # versus vehicle-treated lean mice; * versus vehicle-treated ob mice. (d) Superoxide generation in bone marrow cells is shown to be increased in obese mice and is lowest in the mice treated with CoPP implying an antioxidant effect of HO. Please note bars on the graphs represent the mean ± SEM of four independent experiments; * obese versus vehicle lean; # versus vehicle-treated obese.
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